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Services

Deposits and distribution of strains

The Microbiological Collections of Paraná Network (CMRP-Taxonline) continuously expands its collection of fungal and bacterial strains derived from academic research projects of clinical, industrial, and environmental importance. Deposit requests requires prior analysis by the CMRP curator and technical team to verify compliance with the scope of the collection and biosafety levels. The CMRP collection is addressed to microbial strains of clinical importance with a broad public health impact of human and environmental clinical origin, biotechnological and taxonomic application (reference strains and type strains) and contains biological material restricted to risk groups 1 and 2 (according to the WHO classification). GMOs are not accepted for deposit.

The strains preservation is carried out using freeze-drying, deep-freezing, mineral oil and water (Castellani) methods, in accordance with internationally recommended standards. Its quality is ensured through authentication, feasibility, and purity tests, which are routinely performed in the collection. The authentication of the lineages of the collection is performed by methods of molecular taxonomy associated with conventional taxonomy aiming at confirmation and validation of their identities.

Types of deposit

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Open deposit (public access collection)

Open Deposit (public access collection - SpeciesLink): Comprises biological materials and associated information freely accessible to industry, the academic community, and the external community. This material can be requested by researchers, other collections of the Taxonline network and national and foreign institutions for the purpose of research and specific applications, according to usage rules defined by the Convention on Biological Diversity and by Provisional Measure 2186-16, of August 23, 2001 For cases of cultures that are part of ongoing research, whose publications have not yet been carried out, there is the possibility of restricting them to public access (open deposit with restriction) for up to two years. This request must be made by the depositor via email (colecaocmrp@gmail.com). After this period, the culture will be made available in the database housed in SpeciesLink (splink.cria.org.br).

 

In order to comply with the provisions of Provisional Measure 2186-16, with regard to guaranteeing the sharing of benefits derived from the use of biological material belonging to the national genetic heritage, the depositor must sign the Deposit Form containing the “Authorization for the establishment of TTM ”Allowing CMRP to sign the Material Transfer Term (TTM) with a researcher or institution that is interested in the acquisition and use of its lineage.

Confidential deposit

CMRP also receives biological material for security deposits, of a confidential nature, upon prior request by email. Both the material and the associated information are processed and stored under confidentiality and kept independently from the collection of the public collection.

Important: the deposit of a crop at the CMRP can only be completed after filling out the Deposit form with strains profile it is mandatory to resend the original form via mail. To this end, the applicant must send an email to the collection curator, Dr. Vânia Aparecida Vicente (vaniava63@gmail.com) and to the collection's technical manager, Ms. Kariman Bochnie (colecaocmrp@gmail.com), containing the deposit request, the origin of the biological material to be deposited, and a brief justification for the deposit at the CMRP.

Culture distribution

CMRP provides microbial strains to public and private institutions that have infrastructure and specialized personnel. For safety and public health reasons, strains cannot be sent to private addresses.

 

In the case of the requested culture belonging to the national genetic heritage, the Material Transfer Term (TTM) must be signed by the applicant and returned to the CMRP by mail.

 

In specific cases of biological risk 2, the request must include a brief explanation of the intended use for the requested material, signed by the applicant and / or qualified professional authorized. The “Applicant's agreement of agreement for risk group 2 (TCS) assuming the risks and responsibilities associated with receiving, storing, and using the microorganisms / material in question. The CMRP will verify that the applicant complies with the necessary rules to receive and handle the biohazard microorganism 2. The culture will be sent by courier, in appropriate packaging, and the costs will be borne by the applicant. The lineage may also be removed personally by the applicant.

 

The CMRP cultures strains will be distributed in the form of lyophilized ampoules, or alternatively, in the form of active peaks.

 

The acquisition of CMRP cultures available for public access can only be made after signing the TTM. In case the culture belongs to risk group 2, the applicant's agreement term - TCS, must also be signed.

 

To request to remove a culture, please contact us by email: colecaocmrp@gmail.com

Molecular characterization and independent culture methods

CMRP provides services for the characterization and identification of microorganisms (bacteria, actinobacteria, filamentous fungi and yeasts), based on the integration of molecular taxonomy data (DNA sequencing and genetic distance analysis) and conventional taxonomy data (morphological characteristics). Independent culture methods are used to characterize microbial diversity in different types of samples, using molecular biology and metagenomics analysis. These approaches include the analysis of nucleic acids extracted directly from samples under study and libraries of total DNA and rRNA with 18S, 16S and ITS markers, in order to assess phylogenetic diversity and its relationship with diversity of functions; identify community-coded functions and effects on the ecosystem; and explore the potential of technological applications.

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Taxonomic characterization and / or identification can only be carried out after signing the Term of Deposit. To do so, the client must send an email to colecaocmrp@gmail.com or to the collection curator, Dr. Vânia Aparecida Vicente (vanivava63@gmail.com), containing the service request of interest.

 

After receiving the signed document, we will be able to receive the culture (s) for identification. About shipping: the culture may be in a disposable plate or Falcon tube, provided they are well packed in bubble wrap.

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Training, collaborations and technical-scientific consultansy

CMRP present several routine procedures, ranging from isolation and identification of microorganisms, to molecular characterization. These routine procedures at our center are also offered as courses, in addition to being available for collaborations and scientific technical consultancy.

 

The request for these services can be made by e-mail to colecaocmrp@gmail.com